Biolegend, 156541, Brilliant Violet 711™ anti-mouse NK-1.1 Antibody, 50μg

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo . NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse NK1.1-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone S17016D cross-blocks anti-mouse NK1.1 clone PK136, and can stain for NK1.1 post-formaldehyde and methanol-based fixation based on in-house testing.
RRID: AB_3097465 (BioLegend Cat. No. 156541)
Structure: NKR-P1 gene family
Distribution: NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)
Function: NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type: NK cells, NKT cells
Biology Area: Immunology, Innate Immunity
Antigen References: Lanier LL. 1997. Immunity 6:371. Yokoyama WM, Seaman WE. 1993. Annu. Rev. Immunol. 11:613. Koo GC, et al. 1986. J. Immunol. 137:3742. Giorda R, Trucco M. 1991. J. Immunol. 147:1701.
Gene ID: 17059
UniProt: View information about NK-1.1 on UniProt.org
Clone: S17016D
Regulatory Status: RUO
Other Names: NKR-P1C, NKR-P1B, Ly-55, CD161b, CD161c
Isotype: Mouse IgG2a, κ