Biolegend, 157102, Purified F(ab')2 Goat anti-mouse IgM (μ chain) Antibody, 500μg

Poly21571 reacts with mouse IgM. It can be used in functional assays, such as B cell activation. It has minimum cross-reactivity with bovine proteins. The optimal working concentration should be determined empirically based on the specific assay of interest.
500μg
Verified Reactivity: Mouse
Antibody Type: Polyclonal
Host Species: Goat
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative
Preparation: The antibody was purified from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. The solution contains no preservative; handle under aseptic conditions.
Application: Activ - Quality tested
Recommended Usage: This product was QC tested by stimulating C57BL/6 splenic B cells. The recommended working concentration is 10 - 20 µg/mL
Product Citations: Wu Q, et al. 2023. J Virol. e0065623:97. PubMed Elliot TAE, et al. 2022. Discov Immunol. 1:kyac009. PubMed
RRID: AB_2814087 (BioLegend Cat. No. 157102)
Cell Type: B cells
Biology Area: Immunology, Signal Transduction
Molecular Family: Adaptor Proteins
Gene ID: 16019
Clone: Poly21571
Regulatory Status: RUO
Isotype: Goat Polyclonal Ig