Biolegend, 157702, Purified anti-mouse EOMES Antibody, 100μg

Eomesodermin, or EOMES, is a transcription factor in the T-box family. During embryonic development, EOMES is crucial in regulating trophoblast differentiation, gastrulation, mesoderm delamination, and development of the cerebral cortex. In the immune system, EOMES is involved in the activation, migration, and differentiation of CD8 + T cells. In cooperation with another T-box transcription factor, T-bet, EOMES induces production of IFN-γ and enhances cytotoxic activities of effector CD8 + T cells. EOMES has been shown to be required to maintain long-term memory of CD8 + T cells and is important for homeostasis of memory and effector T cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse EOMES recombinant protein (463-707 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested WB, IP, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: W17001A was tested for ICC using 4% PFA-fixed HeLa (negative control) and NK-92 (positive control cells) permeabilized with either Triton X-100 or methanol. Both methods resulted in high non-specific staining in HeLa cells. We therefore don’t recommend W17001A for this application.
RRID: AB_2876538 (BioLegend Cat. No. 157702)
Distribution: Nucleus
Antigen References: Cooper L, et al. 2018. Plos One. 13(12). Pikovskaya O, et al. 2016. J.Immunol. 196(4):1449-54. Lupar E, et al. 2015. J.Immunol. 195(10):4742-52.
Gene ID: 13813
UniProt: View information about EOMES on UniProt.org
Clone: W17001A
Regulatory Status: RUO
Other Names: Eomesodermin, T-box brain protein 2 (TBR-2), TBR2, TBR-2, TBR 2
Isotype: Rat IgG2b, λ