Biolegend, 157703, Alexa Fluor® 647 anti-mouse EOMES Antibody, 25μg

Eomesodermin, or EOMES, is a transcription factor in the T-box family. During embryonic development, EOMES is crucial in regulating trophoblast differentiation, gastrulation, mesoderm delamination, and development of the cerebral cortex. In the immune system, EOMES is involved in the activation, migration, and differentiation of CD8 + T cells. In cooperation with another T-box transcription factor, T-bet, EOMES induces production of IFN-γ and enhances cytotoxic activities of effector CD8 + T cells. EOMES has been shown to be required to maintain long-term memory of CD8 + T cells and is important for homeostasis of memory and effector T cells.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse EOMES recombinant protein (463-707 a.a.) expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: W17001A was tested for ICC using 4% PFA-fixed HeLa (negative control) and NK-92 (positive control cells) permeabilized with either Triton X-100 or methanol. Both methods resulted in high non-specific staining in HeLa cells. We therefore don’t recommend W17001A for this application.
Product Citations: Wang S, et al. 2022. Sci Adv. 8:eabn3883. PubMed Campisi L, et al. 2022. Nature. 606:945. PubMed Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed Reyes RM, et al. 2021. Oncoimmunology. 10:2006529. PubMed
RRID: AB_2814093 (BioLegend Cat. No. 157703)
Distribution: Nucleus
Antigen References: Cooper L, et al. 2018. Plos One. 13(12). Pikovskaya O, et al. 2016. J.Immunol. 196(4):1449-54. Lupar E, et al. 2015. J.Immunol. 195(10):4742-52.
Gene ID: 13813
UniProt: View information about EOMES on UniProt.org
Clone: W17001A
Regulatory Status: RUO
Other Names: Eomesodermin, T-box brain protein 2 (TBR-2), TBR2, TBR-2, TBR 2
Isotype: Rat IgG2b, λ