Biolegend, 157907, Alexa Fluor® 647 anti-mouse CD218a (IL-18Rα) Antibody, 25μg

The IL-18 receptor (IL-18R) complex is composed of two known chains, IL-18 receptor α (IL-18Rα) and IL-18 receptor β (IL-18Rβ). IL-18Rα (IL-1R5) is an IL-1 receptor super family member. It was originally described as IL-1 receptor-related protein (IL-1Rrp) because of its homology with the IL-1/Toll receptor family. IL-18Rα is the extracellular binding domain of the IL-18 receptor complex. It is expressed on the majority of peripheral CD4 + T cells, Th1 cells, and reciprocally repressed during Th2 development. The expression of IL-18Rα is induced by an IL-12/Stat4 pathway and inhibited by IL-4/Stat6 pathway. Binding of IL-18Rα together with IL-18β to proinflammatory cytokine IL-18 plays an important role in innate and autoimmune responses.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD218a
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Product Citations: Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
RRID: AB_2860736 (BioLegend Cat. No. 157907) AB_2876539 (BioLegend Cat. No. 157908)
Structure: An IL-1 receptor super family member
Distribution: Expressed on T cells, NK cell subset, neutrophils, IL-12 stimulated tonsillar B cells, and intestinal epithelial cells (IEC).
Function: Bind to pro inflammatory cytokine IL-18 and play an important role in innate and autoimmune responses.
Ligand/Receptor: IL-18
Cell Type: B cells, Endothelial cells, Neutrophils, NK cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Parnet P, et al. 1996. J. Biol. Chem. 271:3967. Thomassen E, et al. 1998. J. Interferon Cytokine Res. 18:1077-88. Debets R, et al. 2000. J. Immunol. 165:4950-6. Smeltz RB, et al. 2001. J. Exp. Med. 194:143-53.
Gene ID: 16182
UniProt: View information about CD218a on UniProt.org
Clone: A17071D
Regulatory Status: RUO
Other Names: IL-1 Rrp, CDw218a, IL-18 receptor a
Isotype: Rat IgG2b, κ