Biolegend, 158010, PerCP/Cyanine5.5 anti-mouse CD16 Antibody, 100μg

CD16 also know as Fcgr3 is a 50-65 kD type I transmembrane protein, member of the Fc gamma receptor family and  Ig superfamily. CD16  is expressed  on most myeloid cells including monocytes, macrophages, dendritic cells, and is also expressed by NK cells and NKT cells. CD16 is involved in cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC); its ligands are IgG1, IgG2a and IgG2b.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD16 - transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. Clone S17014E does not cross-react with CD32 or CD64.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Anti-mouse CD16/32 clones 93 and 2.4G2 both block clone S17014E staining.
RRID: AB_2876542 (BioLegend Cat. No. 158009) AB_2876542 (BioLegend Cat. No. 158010)
Structure: Type I transmembrane protein, 50-65 kDa; member of the Fc gamma receptor family and Ig superfamily
Distribution: Expressed on most myeloid cells including monocytes, macrophages, dendritic cells; also on NK cells and NKT cells
Function: Fc receptor, involved in cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
Ligand/Receptor: IgG1, IgG2a and IgG2b
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Nimmerjahn F1 & Ravetch JV. 2008. Nat Rev Immunol. 8(1):34-47. Biburger M & Nimmerjahn F. 2012. Immunol Lett. 143(1):53-9. Arase N, et al. 2003. J Immunol. 170:3054.
Gene ID: 14131
UniProt: View information about CD16 on UniProt.org
Clone: S17014E
Regulatory Status: RUO
Other Names: Fcgr3, Fc receptor, IgG, low affinity III, Fc gamma receptor, Fc gamma receptor 3
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.