Biolegend, 158027, Brilliant Violet 605™ anti-mouse CD16 Antibody, 50μg

CD16 also know as Fcgr3 is a 50-65 kD type I transmembrane protein, member of the Fc gamma receptor family and  Ig superfamily. CD16  is expressed  on most myeloid cells including monocytes, macrophages, dendritic cells, and is also expressed by NK cells and NKT cells. CD16 is involved in cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC); its ligands are IgG1, IgG2a and IgG2b.
50μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD16 - transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Anti-mouse CD16/32 clones 93 and 2.4G2 both block clone S17014E staining.
RRID: AB_3097611 (BioLegend Cat. No. 158027)
Structure: Type I transmembrane protein, 50-65 kDa; member of the Fc gamma receptor family and Ig superfamily
Distribution: Expressed on most myeloid cells including monocytes, macrophages, dendritic cells; also on NK cells and NKT cells
Function: Fc receptor, involved in cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
Ligand/Receptor: IgG1, IgG2a and IgG2b
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Nimmerjahn F1 & Ravetch JV. 2008. Nat Rev Immunol. 8(1):34-47. Biburger M & Nimmerjahn F. 2012. Immunol Lett. 143(1):53-9. Arase N, et al. 2003. J Immunol. 170:3054.
Gene ID: 14131
UniProt: View information about CD16 on UniProt.org
Clone: S17014E
Regulatory Status: RUO
Other Names: Fcgr3, Fc receptor, IgG, low affinity III, Fc gamma receptor, Fc gamma receptor 3
Isotype: Rat IgG2a, κ