Biolegend, 158218, PE/Fire™ 744 anti-mouse CD127 (IL-7Rα) Antibody, 100μg

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse IL7R-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 744 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. True-Stain Monocyte Blocker™ (Cat No. 426102) is recommended to minimize non-specific staining of PE/Fire™ 744 on monocytes and macrophages. * PE/Fire™ 744 has a maximum excitation of 565 nm and a maximum emission of 744 nm.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone S18006K is able to completely block the binding of clone SB/199 and partially block the binding of clone A7R34 to IL-7Rα.
RRID: AB_3675008 (BioLegend Cat. No. 158217) AB_3675008 (BioLegend Cat. No. 158218)
Structure: Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes
Biology Area: Immunology, Signal Transduction
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Sudo T, et al. 1993. Proc Natl Acad Sci USA. 90:9125. Okuno Y, et al. 2002. Proc Natl Acad Sci USA. 99:6246. Pillai M, et al. 2004. Leuk Lymphoma. 45:2403.
Gene ID: 16197
UniProt: View information about CD127 on UniProt.org
Clone: S18006K
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Rat IgG2b, λ