Biolegend, 158603, PE anti-mouse IRF5 Antibody, 25μg

The interferon regulatory factor (IRF) family of transcription factors comprises nine members with conserved domains. IRF5 mediates the induction of proinflammatory cytokine, such as IL-6 and TNFa. IRF5 is highly expressed in monocytes, macrophages, B cells, and dendritic cells. Expression of IRF5 is associated with many autoimmune diseases.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse IRF5 recombinant protein (149-400 a.a.)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This clone was tested for ICC using RAW264.7 cells fixed with 4% PFA and permeabilized with either Triton X-100 or methanol. While both methods were compatible with IRF5 staining, the signal was weak and did not meet our sensitivity standards.This clone does not recognize human IRF5. For detection of human IRF5, please use BioLegend clone 11F4A09 (Cat. No. 652602).During product development testing of this clone for western blot, a ~37 kD protein of unknown origin was faintly detected. This protein may represent a minor IRF5 degradation product.
Product Citations: Goswami S, et al. 2021. J Cell Physiol. 236:8148. PubMed Wang Y, et al. 2022. iScience. 25:104453. PubMed Ganguly S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:891. PubMed
RRID: AB_2819919 (BioLegend Cat. No. 158603) AB_2876549 (BioLegend Cat. No. 158604)
Structure: 497 a.a. (56 kD)
Distribution: Monocytes, macrophages, B cells, dendritic cells
Function: Involved in IFNa and IFNb induction in response to viral infection
Cell Type: B cells, Macrophages, Monocytes
Biology Area: Immunology, Signal Transduction
Molecular Family: Adaptor Proteins, Immune Checkpoint Receptors
Antigen References: Kobayashi S, et al. 2019. J Immunol. 203(6):1447-1456. Nie S, et al. 2019. Clin Immunol. 207:24-35. Pandey SP, et al. 2019. Mucosal Immunol. 12(4):1065.
Gene ID: 27056
UniProt: View information about IRF5 on UniProt.org
Clone: W16007B
Regulatory Status: RUO
Other Names: IRF5, Irf5, IRF-5, Interferon regulatory factor 5
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.