Biolegend, 159212, PerCP/Cyanine5.5 anti-mouse CD86 Antibody, 100μg

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD86
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone A17199A is able to completely block the binding of clone PO3 to CD86.
RRID: AB_3106039 (BioLegend Cat. No. 159211) AB_3106039 (BioLegend Cat. No. 159212)
Structure: Ig superfamily, 80 kD
Distribution: B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
Function: T cell costimulation, Ig class-switching, NK cell cytotoxicity
Interaction: Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. Hathcock KS, et al. 1993. Science 262:905. Freeman GJ, et al. 1993. Science 262:907. Carreno BM and Collins M. 2002. Annu. Rev. Immunol. 20:29.
Gene ID: 16182
UniProt: View information about CD86 on UniProt.org
Clone: A17199A
Regulatory Status: RUO
Other Names: B7-2, B70, Ly-58
Isotype: Rat IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.