Biolegend, 159408, PE/Cyanine7 anti-mouse IL-13 Antibody, 100μg

Mouse IL-13 was initially cloned from cDNA libraries of activated T cells and was designated as P600. IL-13 is an immunoregulatory cytokine secreted predominantly by activated Th2 cells, and it is a key mediator in the pathogenesis of allergic inflammation. IL-13 shares many functional properties with IL-4, and they share a common receptor subunit, the alpha subunit of the IL-4 receptor (IL-4Ra). IL-13 mediates its effects by interacting with a complex receptor system comprised of IL-4Ra and two IL-13 binding proteins, IL-13Ralpha1 and IL-13Ralpha2. Ligation of the IL-13 receptor complex results in signaling via the insulin receptor substrate (IRS)-1 and 2 and STAT-6 pathways. IL-13 has been implicated in inflammatory responses, such as airway hypersensitivity and mucus hypersecretion, inflammatory bowel disease, and parasitic nematode expulsion.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse IL-13
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC – Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2927809 (BioLegend Cat. No. 159407) AB_2927809 (BioLegend Cat. No. 159408)
Structure: Cytokine
Distribution: Th2 cells, basophils, mast cells, activated eosinophils and NK cells
Function: IL-13 modulates mouse monocytes/macrophages and B cells. IL-13 induces an increase of MHC class II expression in mouse monocytes/macrophages, and direct inhibition of inflammatory cytokines such as TNF-α and IL-1β.
Ligand/Receptor: IL-4Rα, IL-13Rα1, and IL-13Rα2
Cell Type: Basophils, Eosinophils, Mast cells, NK cells, Th2
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: Brown KD, et al. 1989. J Immunol. 142:679-87. McKenzie AN, et al. 1993. Proc Natl Acad Sci USA. 90:3735-9. McKenzie GJ, et al. 1999. J Exp Med. 189:1565-72.
Gene ID: 16163
UniProt: View information about IL-13 on UniProt.org
Clone: W17010B
Regulatory Status: RUO
Other Names: Interleukin 13
Isotype: Rat IgG1, κ