Biolegend, 160004, PE anti-mouse CD287 (TLR7) Antibody, 100μg

Toll-like receptor (TLR) 7, also known as CD287 is a type 1 transmembrane protein, member of the TLR receptor family. TLR7 is expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes and plays an important role in the innate immune response by binding single-stranded RNA (ssRNA). TLR7 signals through MyD88, which leads to the activation and secretion of IFN-α/β and other inflammatory cytokines.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ba/F3 cells expressing flag-6xHis-conjugated mouse TLR7
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Kanno A, et al. 2013. Int Immunol. 25:413
Product Citations: Mulder EJ, et al. 2024. Front Immunol. 1340384:15. PubMed Guenther C, et al. 2024. Front Immunol. 1434030:15. PubMed Raiten J, et al. 2024. Front Pharmacol. 1471563:15. PubMed
RRID: AB_2860749 (BioLegend Cat. No. 160003) AB_2876562 (BioLegend Cat. No. 160004)
Structure: Type 1 transmembrane protein, member of the Toll-like receptor (TLR) family
Distribution: Myeloid and plasmacytoid dendritic cells, and B lymphocytes
Function: Pattern recognition receptor that detects single-stranded RNA, induces proinflammatory cytokine production
Ligand/Receptor: Single-stranded RNA
Cell Type: B cells, Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Innate Immune Signaling
Antigen References: Majer O, et al. 2019. Nature. 575:371. Akilesh HM, et al. 2019. Science 363(6423) Hemmi H, et al. 2002. Nat. Immunol. 3:196.
Gene ID: 170743
UniProt: View information about CD287 on UniProt.org
Clone: A94B10
Regulatory Status: RUO
Other Names: TLR7, Toll-like receptor 7, CD287
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.