Biolegend, 161223, PerCP/Fire™ 780 anti-mouse CD62L Antibody, 25μg

CD62L is a 74-95 kD glycoprotein also known as L-selectin. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse CD62L recombinant protein (39-332)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 780 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Fire™ 780 has a maximum excitation of 478 nm and a maximum emission of 780 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_3675015 (BioLegend Cat. No. 161223) AB_3675015 (BioLegend Cat. No. 161224)
Structure: CD62L is a type I transmembrane glycoprotein with 74-95 kD.
Distribution: CD62L is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells.
Function: CD62L plays roles in lymphocyte homing to lymphoid organs and inflammatory leukocyte trafficking.
Ligand/Receptor: CD34, GlyCAM-1, MAdCAM-1
Cell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Rosen S. 2004. Annu. Rev. Immunol. 22:129 2. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
Gene ID: 20343
UniProt: View information about CD62L on UniProt.org
Clone: W18021D
Regulatory Status: RUO
Other Names: L-selectin
Isotype: Rat IgG2a, κ