Biolegend, 162103, PE anti-mouse CD25 Recombinant Antibody, 25μg

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα. CD25 is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.
25μg
Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This clone binds to mouse CD25, also known as the low affinity IL-2Rα.
RRID: AB_2894644 (BioLegend Cat. No. 162103) AB_2894644 (BioLegend Cat. No. 162104)
Structure: CD25 is a 55 kD glycoprotein.
Distribution: B cells, T cells, Thymocytes, Tregs
Function: IL-2 receptor
Ligand/Receptor: IL-2
Cell Type: B cells, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann TA. 1991. J. Biol. Chem. 266:2681. 3. Read S, et al. 2000. J. Exp. Med. 192:295. 4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.
Gene ID: 16184
UniProt: View information about CD25 on UniProt.org
Clone: QA19A49
Regulatory Status: RUO
Other Names: IL-2Rα, Ly-43, p55, and Tac
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.