Biolegend, 162304, PE anti-mouse CD8a Antibody, 100μg

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD8a
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2894434 (BioLegend Cat. No. 162303) AB_2894434 (BioLegend Cat. No. 162304)
Structure: Ig superfamily, CD8α chain, 34 kD
Distribution: Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells
Function: Co-receptor for TCR
Ligand/Receptor: MHC class I molecule
Cell Type: Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP) 2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete) 3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP) 4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block) 5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) 6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC) 7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC) 8. Kamimura D, et al. 2006. J. Immunol. 177:306. 9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete) 10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed 11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed 12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590
Gene ID: 12525
UniProt: View information about CD8alpha on UniProt.org
Clone: S18018E
Regulatory Status: RUO
Other Names: T8, Lyt2, Ly-2
Isotype: Rat IgG2b, λ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.