Biolegend, 163404, TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) Recombinant Antibody, 500μg

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors and is more effective than TruStain FcX™.
500μg
Verified Reactivity: Mouse
Antibody Type: Recombinant
Host Species: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ PLUS (Human IgG4 anti-mouse CD16/32) for 5 - 10 minutes, on ice, at 0.25 µg per 106 cells in a volume of 100 µL, prior to immunostaining. It is not necessary to wash the cells between the blocking and immunostaining steps.
Product Citations: Xu H, et al. 2023. J Biomed Res. 340:37. PubMed
RRID: AB_2922498 (BioLegend Cat. No. 163403) AB_2922498 (BioLegend Cat. No. 163404)
Structure: Ig superfamily, 40-60 kD
Distribution: B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells
Ligand/Receptor: IgG
Cell Type: B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Gene ID: 1413014131
Clone: QA19A30
Regulatory Status: RUO
Other Names: CD16, CD32
Isotype: Human IgG4, κ