Biolegend, 164702, Purified anti-mouse CD8a Antibody, 100μg

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse CD8a
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-F - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2910346 (BioLegend Cat. No. 164702)
Structure: Ig superfamily, CD8α chain, 34 kD
Distribution: Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells
Function: Co-receptor for TCR
Ligand/Receptor: MHC class I molecule
Cell Type: Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Ledbetter JA, et al. 1979. Immunol Rev. 47:63-90. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. Takahashi K, et al. 1992. Proc Natl Acad Sci USA. 89:5557-61. Ledbetter JA, et al. 1981. J Exp Med. 153:1503-16. Hata H, et al. 2004. J Clin Invest. 114:582-8. Fan WY, et al. 2001. Exp Biol Med. 226:1045-50. Shih FF, et al. 2006. J Immunol. 176:3438-48. Kamimura D, et al. 2006. J Immunol. 177:306-14. Bouwer HGA, et al. 2006. Proc Natl Acad Sci USA. 103:5102-7. Kao C, et al. 2005. Int Immunol. 17:1607-17. Ko SY, et al. 2005. J Immunol. 175:3309-17. Rasmussen JW, et al. 2006. Infect Immun. 74:6590-8.
Gene ID: 12525
UniProt: View information about CD8a on UniProt.org
Clone: S18018A
Regulatory Status: RUO
Other Names: T8, Lyt2, Ly-2
Isotype: Rat IgG1, κ