Biolegend, 165102, Purified anti-mouse CD294 (CRTH2) Antibody, 100μg

CD294, also known as CRTH2, is a seven-transmembrane protein coupled with heterotrimeric G proteins. CRTH2 is the prostaglandin D2 receptor and is expressed by Th2 cells, eosinophils, and basophils. CD294 prevents the apoptosis of Th2 cells and mediates the chemotaxis of CRTH2 expressing cells to the sites of allergic inflammation, such as the asthmatic lung.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse CD294 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC – Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2924496 (BioLegend Cat. No. 165102)
Structure: Seven-transmembrane protein coupled with G proteins
Distribution: Th2 cells, eosinophils, basophils
Function: Chemotaxis of eosinophils, basophils and Th2 cells, prevention of apoptosis in Th2 cells
Interaction: Heterotrimeric G proteins
Ligand/Receptor: Prostaglandin D2
Cell Type: Basophils, Eosinophils, Th2
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family: CD Molecules
Antigen References: Abe H, et al. 1999. Gene. 227:71-7. Hata AN, et al. 2003. J Pharmacol Exp Ther. 306:463 Luster AD and Tager AM. 2004. Nat Rev Immunol. 4:711-24
Gene ID: 14764
UniProt: View information about CD294 on UniProt.org
Clone: S17004A
Regulatory Status: RUO
Other Names: Chemoattractant receptor-homologous molecule expressed on Th2 cells, GPR44, G-protein coupled receptor 44, DP2
Isotype: Mouse IgG2a, κ