Biolegend, 165706, PE anti-mouse CD52 Antibody, 100μg

CAMPATH-1 antigen, also known as cluster of differentiation 52 (CD52), is a glycoprotein that in humans is encoded by the CD52 gene. It is expressed in the embryo mesenchyme and thymus primordium. CD52 acts upstream of or within response to bacterium and is located in the sperm midpiece. CD52 is present on the surface of mature lymphocytes, but not on the stem cells from which these lymphocytes were derived. It also is found on monocytes and dendritic cells. Furthermore, it is found within the male genital tract and is present on the surface of mature sperm cells. Due to its highly negative charge and presence on sperm cells and lymphocytes, it has been thought that CD52 functions as an anti-adhesion since it allows cells to freely move around.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3662205 (BioLegend Cat. No. 165705) AB_3662205 (BioLegend Cat. No. 165706)
Structure: CD52 is a peptide of 12 amino acids, anchored to glycosylphosphatidylinositol (GPI).
Distribution: Expressed on lymphohematopoietic tissues, including thymus, spleen, and bone marrow, but not in liver, kidney, and brain.
Function: Promote cell-mediated killing (antibody-dependent cellular cytotoxicity)
Cell Type: B cells, Eosinophils, Erythrocytes, Macrophages, Monocytes, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Gudrun R, et al. 2002. Blood. 101:1422.
Gene ID: 23833
UniProt: View information about CD52 on UniProt.org
Clone: S20005A
Regulatory Status: RUO
Other Names: CAMPATH-1 antigen
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.