Biolegend, 165802, Purified anti-mouse Arginase 1 Antibody, 100μg

ARG1 (Arginase 1), is a marker for the M2 anti-inflammatory macrophage subset that hydrolyzes l-arginine into urea and ornithine, and plays an important role in wound healing, antiviral immune responses, and atherosclerosis. The inherited deficiency of this enzyme results in argininemia, which is an autosomal recessive disorder characterized by hyperammonemia. Recent studies show that intracellular pathogens induce the expression of ARG1 in mouse macrophages through the Toll-like receptor (TLR) pathway. The deletion of this enzyme in macrophages favors host survival. Other studies indicate that ARG1 also plays a role in T cell responses and selective antagonists of ARG1 might prove beneficial in fighting diseases in which T-cell responses are inappropriately suppressed.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli expressed protein aa 1-323
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone W21047I does not block the staining of reference clone A1eXF5 on the target cells (BMDM).
RRID: AB_3068107 (BioLegend Cat. No. 165802)
Structure: 322 amino acids with a predicted molecular weight of approximately 35 kD
Distribution: Express on dendritic cells & macrophages. Expressed predominantly in the liver. It can also be induced in macrophages. It is cytoplasmic target.
Function: Arginase 1 plays an important role in processes such as wound healing, antiviral immune response, and atherosclerosis.
Interaction: Binds 2 manganese ions per subunit as a cofactor
Ligand/Receptor: l-arginine
Cell Type: Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Enzymes and Regulators
Antigen References: Wu G, et al. 1998. Biochem J. 336:1. El Kasmi KC, et al. 2008. Nat Immunol. 9:1399-406. Pesce JT, et al. 2009. PLoS Pathog. 5:e1000371. Burrack KS, et al. 2014. Front Immunol. 5:428. Pourcet B, et al. 2013. Trends Cardiovasc Med. 23:143-52. Bronte V, et al. 2003. Trends Immunol. 24:302-6.
Gene ID: 383
UniProt: View information about Arginase 1 on UniProt.org
Clone: W21047I
Regulatory Status: RUO
Other Names: Arginase 1, liver type Arginase, Type I Arginase, ARG1
Isotype: Rat IgG2b, κ