Biolegend, 167202, Purified anti-mouse CCL17 (TARC) Antibody, 100μg

CC chemokine ligand 17 (CCL17), also known as Thymus and Activation-Regulated Chemokine (TARC), was initially identified as a T cell chemokine expressed constitutively in the thymus and by PBMCs upon activation.  CCL17 has since been found to be secreted by a variety of cell types and elevated in multiple inflammatory and autoimmune conditions. CCL17-mediated recruitment of Th2 and CLA + CD4 + T cells plays a key role in allergic diseases such as atopic dermatitis, allergic asthma, allergic rhinitis, and allergic contact dermatitis. In addition, CCL17 is significantly elevated in cases of idiopathic pulmonary fibrosis. CCL17 + DCs accumulate in atherosclerotic lesions where they are thought to limit Treg expansion. DC-derived CCL17 along with CCL22 is also implicated in the recruitment of Tregs to inflammatory sites in chronic hepatitis.  CCL17 is upregulated by TNFα, IL-4, and IFNγ in bronchial epithelial cells, TGFβ1, TNFα, and IFNγ in a human keratinocyte cell line, and histamine and prostaglandin E2P in immature DCs.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse Recombinant CCL17 (aa24-93)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC – Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3698938 (BioLegend Cat. No. 167202)
Structure: Chemokine
Distribution: Upon activation, CCL17 is expressed by PBMCs, monocytes, dendritic cells, bronchial epithelial cells, smooth muscle cells, keratinocytes, and fibroblasts.
Function: CCL17 chemoattracts T cells, Th2 cells, and CLA+ CD4+ T cells. It plays a major role in allergic diseases. CCL17 can be upregulated by IL-4 in T cells and by a combination of cytokines in different cells.
Interaction: T cells, Th2 cells, CLA+ CD4 T cells, T (reg) cells, NK cells, NKT cells, and macrophages
Ligand/Receptor: CCR4 (CD194)
Cell Type: Dendritic cells, Epithelial cells, Fibroblasts, Monocytes
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: Imai T, et al. 1997. J. Biol. Chem. 272:15036. McIlroy A, et al. 2006. Immunology 117:507. Yogo Y, et al. 2009. Respir. Res. 10:80. Stutte S, et al. 2010. P. Natl. Acad. Sci USA 107:8736. Oo YH, et al. 2010. J. Immunol. 184:2886. Weber C, et al. 2011. J. Clin. Invest. 121:2898
Gene ID: 20295
UniProt: View information about CCL17 on UniProt.org
Clone: A20096A
Regulatory Status: RUO
Other Names: CC chemokine TARC, C-C motif chemokine 17, CCL17, chemokine (C-C motif) ligand 17, small inducible cytokine subfamily A (Cys-Cys), member 17, Small-inducible cytokine A17, T cell-directed CC chemokine, TARC, Thymus and activation-regulated chemokine
Isotype: Rat IgG2b, κ