Biolegend, 200002, Purified anti-rat CD3 Recombinant Antibody, 100μg

CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.
100μg
Verified Reactivity: Rat
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: F344 rat spleen cells stimulated with PMA and calcium ionophore
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Wang Q, et al. 2024. Cell Rep Med. 101357:5. PubMed
RRID: AB_2910354 (BioLegend Cat. No. 200002)
Structure: Ig superfamily, approximately 20-25 kD
Distribution: Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC complex
Cell Type: NKT cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Tanaka T, et al. 1989. J Immunol. 142:2791-5. Elbe A, et al. 1993. J Invest. Dermatol. 102:74-9.
Gene ID: 2571030067831560925300
UniProt: View information about CD3 on UniProt.org
Clone: QA19A03
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Mouse IgG2a, κ