Biolegend, 200502, Purified anti-rat CD252 (OX-40 Ligand) Antibody, 500μg

OX-40 Ligand (OX-40L) is a 22 kD member of the TNF superfamily (TNFSF4), also known as CD252 or CD134L. It is expressed on dendritic cells, peritoneal macrophages, and activated B cells. The interaction of OX-40L with OX-40 (CD134) is important for B cell antibody production and T cell activation and proliferation. The ATM-2 antibody is able to block the costimulatory function of OX-40L.
500μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: ACI-S1 rat T-cell lymphoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: blocking the binding of OX-40 to OX-40L and the inhibition of T cell costimulatory activity of OX40L1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 200506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 200504) or biotinylated anti-mouse IgG (Cat. No. 405303) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200508).
Application References(PubMed link indicates BioLegend citation): Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041.
RRID: AB_2571881 (BioLegend Cat. No. 200501)
Structure: TNF superfamily, 22 kD
Distribution: Dendritic cells, activated B cells
Function: T cell dependent humoral responses
Ligand/Receptor: OX40 (CD134)
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Satake, Y., et al. 2000. Biochem. Biophys. Res. Commun. 270:1041. 2. Akiba, H., et al. 1998. Biochem. Biophys. Res. Commun. 251:131.3. Akiba, H., et al. 2000. J. Exp. Med. 191:375.
Gene ID: 22164
UniProt: View information about CD252 on UniProt.org
Clone: ATM-2
Regulatory Status: RUO
Other Names: OX-40 Ligand, CD134L, TNFSF4
Isotype: Mouse IgG1, κ