Biolegend, 200802, Purified anti-rat TSPAN8 Antibody, 100μg

Tetraspanins, also referred to as the transmembrane 4 superfamily (TM4SF) proteins, have four transmembrane alpha-helices and two extracellular domains. It acts as molecular facilitators forming a web between themselves and a multitude of molecules. It is also involved in adhesion, motility, matrix modulation and cell fusion. Tetraspanins/Tetraspanin-complexes are constitutive components of exosomes. During exosome assembly, Tetraspanins become internalized via a tyrosine-based internalization motif. Internalized membrane microdomains are recruited into early endosomes from where they can become incorporated into multivesicular body (MVB), which migrate towards and fuse with the cell membrane releasing invaginated endosomes as exosomes.
100μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2941445 (BioLegend Cat. No. 200802)
Structure: Four transmembrane alpha-helices and two extracellular domains
Distribution: Multi-pass membrane protein
Function: Adhesion, motility, matrix modulation and cell fusion
Interaction: LIPR1, MEP1B, STX1-2, TIM23, S38A7
Ligand/Receptor: Integrin binding
Biology Area: Immunology
Antigen References: Berditchevsk F, et al. 2001. Journal of Cell Science. 114 (Pt 23): 4143–51. Mu W, et al. 2020. Cells. 9:319.
Gene ID: 171048
UniProt: View information about TSPAN8 on UniProt.org
Clone: 2D9/TSPAN8
Regulatory Status: RUO
Other Names: Tspan8, tetraspanin-8
Isotype: Mouse IgG2a, κ