Biolegend, 203007, PE anti-rat CD152 Antibody, 25μg

CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation.
25μg
Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Lin CH and Hunig T, et al. 2003. Eur. J. Immunol. 33:626. (IP) Nessi V, et al. 2010. J. Immunol. 185:5656. PubMed
RRID: AB_2276898 (BioLegend Cat. No. 203007)
Structure: Ig superfamily, 33 kD
Distribution: Activated T and B cells
Function: Negative regulator of T cell activation
Ligand/Receptor: CD80 (B7-1), CD86 (B7-2)
Cell Type: B cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Allison JP, et al. 1995. Science 270:932. 3. Waterhouse P, et al. 1995. Science 270:985. 4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.
Gene ID: 63835
UniProt: View information about CD152 on UniProt.org
Clone: WKH203
Regulatory Status: RUO
Other Names: Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.