Biolegend, 250304, PE anti-marmoset CD8 Antibody, 100μg

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α 3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
100μg
Verified Reactivity: Common Marmoset
Reported Reactivity: Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Marmoset mononuclear cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The 6F10 antibody has been reported to cross-react with lymphocytes from squirrel monkeys but not from cynomolgus monkeys or humans. Additional reported applications for the relevant formats include: iimmunohistochemical staining of frozen spleen sections1, Western blotting1, immunoprecipitation1.
Application References(PubMed link indicates BioLegend citation): Ito R, et al. 2008. Immunol. Lett. 121:116. (IHC, IP, WB)
Product Citations: Gordeychuk IV, et al. 2019. Acta Naturae. 10:63. PubMed Wang Y, et al. 2021. Front Microbiol. 12:701566. PubMed
RRID: AB_2562122 (BioLegend Cat. No. 250304)
Structure: Ig superfamily, homodimer or heterodimer with CD8β
Distribution: Majority of thymocytes, T cell subset, NK cells
Function: MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor: MHC Class I molecules
Cell Type: NK cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
Gene ID: 100387674
UniProt: View information about CD8 on UniProt.org
Clone: 6F10
Regulatory Status: RUO
Other Names: T8, Leu2
Isotype: Mouse IgG1
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.