Biolegend, 260322, Purified anti-Mcl-1 Antibody, 100tests

Mcl-1 is a member of the pro-survival BCL-2 family of apoptotic regulatory proteins. Mcl-1 plays a crucial role in the survival and development of various cell types by binding to proapoptotic proteins and inhibiting the mitochondrial apoptotic pathway. Unlike Bcl-2 and Bcl-xL, Mcl-1 lacks a BH4 domain and contains an N-terminal PEST region with specific sequences for ubiquitination, phosphorylation, and caspase cleavage sites, which are important in regulating the half-life. Phosphorylation of at T92 and T163 by ERK inhibits Mcl-1 turnover, whereas phosphorylation by GSK3β at S159 facilitates proteosomal degradation. Increased expression of Mcl-1 is correlated with tumor survival and is associated with resistance of chemotherapeutics.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein comprising a portion of the N terminus of human Mcl-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only Fixation/permeabilization with Fixation buffer followed by 0.5 % Triton X-100 is not recommended due to dim signal.
RRID: AB_3720577 (BioLegend Cat. No. 648852) AB_3720577 (BioLegend Cat. No. 648853)
Structure: Mcl-1 is a 350 amino acid protein with a predicted molecular weight of 37.3 kD.
Distribution: Tissue distribution: widely expressed Cellular distribution: primarily mitochondria (outer membrane), also nucleus, cytosol and endoplasmic reticulum
Function: Anti-apoptotic/pro-survival protein
Interaction: Mcl-1 forms a homodimer. Interacts with HIF3A, PMAIP1, BBC3, BAX, BAK1, TPT1, BOP, BCL2L11, BAD, BOK, BIK, and BMF.
Cell Type: Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Antigen References: Kozopas K, et al. 1993. P. Natl. Acad. Sci. USA. 90:3516. Zhou P, et al. 1997. Blood. 89:630. Chao J, et al. 1998. Mol. Cell. Biol. 18:4883. Epling-Burnette P, et al. 2001. J. Immunol. 166:7486. Moulding D, et al. 1998. Blood. 92:2495. Inoshita S, et al. 2002. J. Biol. Chem. 277(46):43740. Opferman JT, et al. 2003. Nature. 426:671.
Gene ID: 4170
UniProt: View information about Mcl-1 on UniProt.org
Clone: W23229E
Regulatory Status: RUO
Other Names: Induced myeloid leukemia cell differentiation protein Mcl-1, Bcl-2-like protein 3 (BCL2-L-3), Bcl-2-related protein EAT/mcl1
Isotype: Rat IgG2a, κ