Biolegend, 260340, GMP PE/Cyanine7 anti-human CD71 Antibody, 100tests

CD71 is a 95 kD type II homodimeric transmembrane glycoprotein also known as T9 and transferrin receptor. It is expressed on proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NALM-6 pre-B cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: 100 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Transferrin receptor family, type II glycoprotein, 95 kD
Distribution: Proliferating cells, reticulocytes, erythroid precursors
Function: Mediates uptake of iron
Ligand/Receptor: Transferrin
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hentze M, et al. 1996. P. Natl. Acad. Sci. USA 93:8175. 2. Trowbridge I, et al. 1993. Annu. Rev. Cell Biol. 9:129.
Gene ID: 7037
UniProt: View information about CD71 on UniProt.org
Clone: CY1G4
Workshop: A015
Other Names: T9, Transferrin receptor
Isotype: Mouse IgG2a, κ