Biolegend, 260358, GMP APC anti-human CD41 Antibody, 100tests

CD41 is a 125/25 kD α subunit of the gpIIb/IIIa (CD41/CD61) complex. CD41 is a heterodimer composed of a heavy chain (gpIIbα) and light chain (gpIIbβ) linked by a single disulfide bond. It is a member of the integrin family primarily expressed on platelets and megakaryocytes. CD41 has been reported to be involved with platelet aggregation and platelet attachment to the ECM. CD41/CD61 complex acts as the receptor for fibrinogen, fibronectin, Von Willebrand factor, and thrombin.
100tests
Reactivity: Human
Reported Reactivity: African Green, Baboon, Capuchin Monkey, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: 25 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and blocking of platelet aggregation2. The HIP8 antibody has been reported to block the activation of platelets by various stimuli, including collagen, and ADP.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. McCarty OJT, et al. 2000. Blood 96:1789. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Zhi L et al. 2013. PLoS One. 8:e79869. (IHC)
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Integrin family, α subunit of CD41/CD61 (GPIIb-IIIa) complex, 125/22 kD
Distribution: Platelets, megakaryocytes
Function: Platelet aggregation, platelet attachment to extracellular matrix
Ligand/Receptor: Fibrinogen, fibronectin, von Willebrand factor, thrombin
Cell Type: Megakaryocytes, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Denzin L, et al. 1996. J. Exp. Med. 184:2153. 2. Denzin L, et al. 1995. Cell 82:155. 3. Riberdy J, et al. 1994. J. Cell Biol. 125:1225.
Gene ID: 3674
UniProt: View information about CD41 on UniProt.org
Clone: HIP8
Workshop: IV P38
Other Names: gpIIb, CD41a
Isotype: Mouse IgG1, κ