Biolegend, 260442, GMP PE anti-human CD1a Antibody, 100tests

CD1a is a 49 kD member of the immunoglobulin superfamily also known as T6 and R4. It is a type I membrane glycoprotein with structural similarities to MHC class I and is non-covalently associated with β2-microglobulin. CD1a plays a role in non-peptide glycolipid antigen presentation to CD1-restricted T cells. It is expressed on cortical double positive and single positive thymocytes, Langerhans cells, and dendritic cells. In addition to antigen presentation, CD1a has been implicated in thymic T cell development.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 200 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported application (for the relevant formats) includes: immunoprecipitation and immunohistochemical staining of frozen sections.2
Application References(PubMed link indicates BioLegend citation): Wood GS, et al. 1983. J. Immunol. 131:212. Facy V, et al. 2005. Toxicol in Vitro. 6:787-95.
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Ig superfamily, MHC I-like molecule, type I transmembrane glycoprotein, 49 kD
Distribution: Cortical thymocytes, Langerhans cells, dendritic cells
Function: Antigen presentation, lymphocyte activation, thymic T cell development
Ligand/Receptor: CD1-restricted TCRs
Cell Type: Dendritic cells, Langerhans cells, Thymocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Wood GS, et al. 1983. J. Immunol. 131:212. 2. Wood GS, et al. 1985. Am. J. Pathol. 120:371. 3. Martin LH, et al. 1987. P. Natl. Acad. Sci. USA 84:9189.
Gene ID: 909
UniProt: View information about CD1a on UniProt.org
Clone: SK9
Other Names: T6, R4, Leu-6
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.