Biolegend, 260470, GMP PE anti-human CD79a (Igα) Antibody, 100tests

CD79a is a 47 kD type I integral membrane protein, also known as mb-1 or Iga. It is a member of the Ig superfamily and disulphide-associated with CD79b (B29). The interaction of CD79a/CD79b heterodimer with B cell suface Ig forms B cell antigen receptor complex. CD79a is expressed in B cells from early pre-B to plasma cell stage. It has been shown that CD79a is also weakly expressed in some precursors of T- and myeloid cells. CD79 mediates the transport of IgM to B cell surface and transduces signals initiated by BCR aggregation.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 12 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: 47 kD, type I integral protein, Ig superfamily, CD79a/CD79b heterodimer interacts with B cell membrane Ig.
Distribution: B cells (from early pre-B to plasma cell stage), some T and myeloid cell precursors
Function: In association with CD79b and B cell membrane Ig to form B cell antigen receptor complex
Cell Type: B cells, Hematopoietic stem and progenitors
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS 2. Astsaturov IA, et al. 1996. Leukemia 10:769 3. Mson DY, et al. 1995 Blood 86:1453 4. Hashimoto M, et al. 2002. J. Pathol. 197:341
Gene ID: 973
UniProt: View information about CD79a on UniProt.org
Clone: HM47
Workshop: V cB017
Other Names: Mb-1, Iga, CD79
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.