Biolegend, 260508, GMP PE anti-human CD203c Antibody, 100tests

CD203c, a transmembrane protein and a member of the ectoenzyme family, is involved in the hydrolysis of extracellular oligonucleotides, nucleoside phosphates, and NAD (possesses ATPase and ATP pyrophosphatase activity). The molecular weight of CD203c is between 130 and 150 kD under reducing conditions and 270 kD under non-reducing conditions. CD203c is expressed on basophils and mast cells, and is highly expressed on activated basophils. Secretory glands in endometrium and glioma cells are also positive. CD203c is a multifunctional ectoenzyme involved in the clearance of extracellular nucleotides whose substrates include nucleoside triphosphates, nucleoside diphosphates, cAMP, and NAD.
100tests
Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HEK-293 cells transfected with human E-NPP3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: 100 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Bühring HJ, et al. 1999. Blood 94:2343. Bühring HJ, et al. 2001. Blood 97:3303. Platz IJ, et al. 2001. Int. Arch. Allergy Immunol. 126:335. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Gernez Y, et al. 2011. Int. Arch. Allergy Immunol. 154:318. (FC) PubMed
Disclaimer: GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include: Batch-to-batch consistency Material traceability Documented procedures Documented employee training Equipment maintenance and monitoring records Lot-specific certificates of analysis Quality audits per ISO 13485:2016 QA review of released products
Structure: Transmembrane ectoenzyme family member involved in the hydrolysis of extracellular oligonucleotides, nucleoside phosphates, and NAD (possesses ATPase and ATP pyrophosphatase activity); reduced molecular weight is 130 and 150 kD, unreduced is 270 kD
Distribution: Basophils and mast cells, highly expressed on activated basophils; secretory glands in endometrium and glioma cells are also positive
Function: Multifunctional ectoenzyme involved in the clearance of extracellular nucleotides
Ligand/Receptor: Substrates include nucleoside triphosphates, nucleoside diphosphates, cAMP, and NAD
Cell Type: Basophils, Mast cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Yano Y, et al. 2003. Int. J. Mol. Med. 12:763. 2. Andoh K, et al. 1999. Biochim. Biophys. Acta. 1446:213.
Gene ID: 5169
UniProt: View information about CD203c on UniProt.org
Clone: NP4D6
Workshop: HLDA8
Other Names: E-NPP3, ENPP3
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.