Biolegend, 300460, Brilliant Violet 605™ anti-human CD3 Antibody, 100tests

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).
Application References(PubMed link indicates BioLegend citation): Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP) Graves J, et al. 1991. J. Immunol. 146:2102. (Activ) Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ) Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC) Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ) Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC) Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC) Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Van Dongen JJM, et al. 1988. Blood 71:603. (WB) Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC) Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed Li J, et al. 2015. Cancer Res. 75:508. PubMed Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG) Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Hu X, et al. 2023. Nat Commun. 14:2020. PubMed Afsahi A, et al. 2023. Front Immunol. 14:1179827. PubMed Lukas H, et al. 2023. National Library of Medicine. . 120(33): e2300343120. PubMed McMurray JL, et al. 2022. Cell Rep. 39:110858. PubMed Taura M, et al. 2019. Proc Natl Acad Sci U S A. 116:2282. PubMed Li H, et al. 2018. PLoS Genet. 14:e1007163. PubMed Hill DL, et al. 2021. Elife. 10:. PubMed Nielsen CM, et al. 2021. Cell Reports Medicine. 2(3):100207. PubMed Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed Iwabuchi R, et al. 2021. Frontiers in Immunology. 12:643040. PubMed Lucas C, et al. 2021. Nature. Online ahead of print. PubMed Vanshylla K, et al. 2022. Cell Host Microbe. 30:69. PubMed Vanuytsel K, et al. 2022. Nat Commun. 13:1103. PubMed Minassian AM, et al. 2021. Med (N Y). 2:701. PubMed Lutter L, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1567. PubMed Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed Wright JA, et al. 2021. Front Immunol. 12:705307. PubMed Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed Farhat AM, et al. 2021. Cell Reports. 35(4):109044. PubMed Adel–Patient K, et al. 2018. Clin Transl Allergy. 8:38. PubMed
RRID: AB_2564379 (BioLegend Cat. No. 300459) AB_2564380 (BioLegend Cat. No. 300460)
Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego. 2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329. 3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.
Gene ID: 916
UniProt: View information about CD3 on UniProt.org
Clone: UCHT1
Regulatory Status: RUO
Workshop: III 471
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ