Biolegend, 300619, PerCP/Cyanine5.5 anti-human CD5 Antibody, 25tests

CD5 is a 67 kD single chain type I glycoprotein also known as Leu-1, Ly-1 and T1. It is a member of the scavenger receptor superfamily found on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-CLL), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions upon binding to ligands such as CD72.
25tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Capuchin Monkey, Common Marmoset, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting2 and immunohistochemical staining of acetone-fixed frozen sections2,5.
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York. Renaudineau Y, et al. 2005. Blood 106:2781. (WB IHC) Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. Saliba AE, et al. 2010. P. Natl. Acad. Sci. USA 107:14524. PubMed Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
Product Citations: Kielbassa K, et al. 2023. Leukemia. 37:1268. PubMed Bolton HA, et al. 2015. J Clin Invest. 3627:125. PubMed Drennan S, et al. 2016. Leukemia. 10.1038/leu.2016.356. PubMed
RRID: AB_893379 (BioLegend Cat. No. 300619) AB_893376 (BioLegend Cat. No. 300620)
Structure: Scavenger receptor superfamily, 67 kD
Distribution: T cells, thymocytes, B cell subset, B cell CLL, peripheral blood dendritic cells
Function: Modulates TCR, BCR signaling, thymocyte maturation, T-B cell interaction
Ligand/Receptor: CD72
Cell Type: B cells, Dendritic cells, Leukemia, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Kipps T. 1988. Adv. Immunol. 47:117. 2. Resnick D, et al. 1993. Trends Biochem. Sci. 19:5. 3. Wood GS, et al. 1992. Am. J. Pathol. 14:789.
Gene ID: 921
UniProt: View information about CD5 on UniProt.org
Clone: UCHT2
Regulatory Status: RUO
Workshop: III 518
Other Names: Leu-1, Ly-1, T1, Tp67
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.