Biolegend, 300821, PE/Fire™ 810 anti-human CD137 (4-1BB) Antibody, 25tests

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD137-Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3662403 (BioLegend Cat. No. 300821)
Structure: TNFR superfamily, type I transmembrane protein, 30 kD
Distribution: Activated T cells
Function: T cell costimulation
Ligand/Receptor: 4-1BB ligand (CD137L)
Cell Type: Lymphocytes, NK cells, NKT cells, T cells
Biology Area: Costimulatory Molecules, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family: CD Molecules
Antigen References: Gruss HJ & Dower SK. 1995. Blood. 85:3378-404. Sica G & Chen L. 2000. Adv Exp Med Biol. 465:355-62. Alderson MR, et al. 1994. Eur J Immunol. 24:2219-27. Schwarz H, et al. 1996. Blood 87:2839-45.
Gene ID: 3604
UniProt: View information about CD137 on UniProt.org
Clone: S18014C
Regulatory Status: RUO
Other Names: 4-1BB, ILA, CDw137, TNFRSF9
Isotype: Mouse IgG1, κ