Biolegend, 302115, Ultra-LEAF™ Purified anti-human CD18 Antibody, 100μg

CD18 is a 90-95 kD type I transmembrane protein also known as integrin β 2 subunit, LFA-1 β subunit, and β 2 integrin. CD18 non-covalently associates with CD11a, CD11b or CD11c. CD18 is expressed on all leukocytes. CD18 and associated α chains function in adhesion and signaling in hematopoietic cells.
100μg
Verified Reactivity: Human
Reported Reactivity: Pig
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality testedBlock - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: inhibition of cell adhesion and migration3,4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 302116) is recommended for functional assays.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto T, et al. 1997. Leucocyte Typing VI. Garland Press. London. Van Epps DE, et al. 1989. J. Immunol. 143:3207. (Block) Meerschaert J, et al. 1994. J. Immunol. 152:1915. (Block) Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200. Sommaggio R, et al. 2012. J. Immunol. 188:2075. PubMed Valenzuela NM, et al. 2013. J. Immunol. 190:6635. PubMed
Product Citations: Sahu SR, et al. 2023. Front Immunol. 1274519:14. PubMed Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed Cremasco F, et al. 2021. PLoS One. 16:e0241091. PubMed
RRID: AB_2814126 (BioLegend Cat. No. 302115) AB_2561483 (BioLegend Cat. No. 302116)
Structure: Integrin, type I transmembrane glycoprotein, associates with CD11a, CD11b, or CD11c, 90-95 kD
Distribution: Leukocytes
Function: Adhesion and signaling in hematopoietic system
Ligand/Receptor: ICAMs
Cell Type: Leukocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Anderson D, et al. 1987. Annu. Rev. Med. 38:175. 2. Springer T. 1994. Cell 76:301.
Gene ID: 3689
UniProt: View information about CD18 on UniProt.org
Clone: TS1/18
Regulatory Status: RUO
Workshop: V AS162
Other Names: Integrin β2 subunit, LFA -1 β subunit, β2 integrin, ITGB2
Isotype: Mouse IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.