Biolegend, 302603, FITC anti-human CD25 Antibody, 25tests

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4 + T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
25tests
Verified Reactivity: Human
Reported Reactivity: Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications include: immunocytochemistry3.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations: Garcia–Perez JE, et al. 2019. Front Immunol. 10:998. PubMed Wang H, et al. 2020. Mol Oncol. 14:991. PubMed Felce SL, et al. 2021. Wellcome Open Res. 5:42. PubMed Metelo AM, et al. 2022. Cancer Res Commun. 2:158. PubMed Donadei C, et al. 2023. J Clin Med. 12:. PubMed Thiramanas R, et al. 2020. Cells. 9: . PubMed Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Gartshteyn Y, et al. 2023. Life Sci Alliance. 6: . PubMed Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed Pauthner MG, et al. 2019. Immunity. 50:241. PubMed Beswick E, et al. 2011. Infect Immun. 79:2737. PubMed Aqel SI, et al. 2021. JCI Insight. 6:e142376. PubMed Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed Turner AW, et al. 2020. ACS Infect Dis. 6:1719. PubMed Grivas A, et al. 2022. Front Immunol. 13:964274. PubMed Hassani M, et al. 2020. Iran Biomed J. 24:81. PubMed Liu H, et al. 2021. J Immunother Cancer. 9:. PubMed Qu K et al. 2017. Cancer cell. 32(1):27-41 . PubMed Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed Grigoriou M, et al. 2021. Cancer Immunol Res. 9:726. PubMed Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed Rhijn I, et al. 2016. Proc Natl Acad Sci U S A. 113: 380 - 385. PubMed Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed Kim ST, et al. 2021. J Immunother Cancer. 9:. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Ryba M, et al. 2011. Cytokine. 55:353. PubMed Jiang J, et al. 2016. Sci Rep. 6: 32320. PubMed Businaro R, et al. 2009. Atherosclerosis. 207:74. PubMed Yost KE, et al. 2019. Nat Med. 25:1251. PubMed Meniailo M, et al. 2017. Int Immunopharmacol. 10.1016/j.intimp.2017.06.023. PubMed
RRID: AB_314273 (BioLegend Cat. No. 302603) AB_314274 (BioLegend Cat. No. 302604)
Structure: Type I transmembrane glycoprotein, 55 kD
Distribution: Activated T cells and B cells, monocytes/macrophages, Treg
Function: Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor: IL-2
Cell Type: B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
Gene ID: 3559
UniProt: View information about CD25 on UniProt.org
Clone: BC96
Regulatory Status: RUO
Workshop: V T-072
Other Names: Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype: Mouse IgG1, κ