Biolegend, 302618, Alexa Fluor® 647 anti-human CD25 Antibody, 100tests

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4 + T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
100tests
Verified Reactivity: Human
Reported Reactivity: Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications include: immunocytochemistry3.
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations: Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed Blomberg OS 2023. Cancer Cell. 41(1):106-123.e10. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Tiittanen M, et al. 2013. PLoS One. 7:78420. PubMed Kilpelainen A, et al. 2022. Front Immunol. 13:815041. PubMed Singh KS, et al. 2021. Nature. 589:597. PubMed Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed
RRID: AB_493046 (BioLegend Cat. No. 302617) AB_493045 (BioLegend Cat. No. 302618)
Structure: Type I transmembrane glycoprotein, 55 kD
Distribution: Activated T cells and B cells, monocytes/macrophages, Treg
Function: Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor: IL-2
Cell Type: B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5. 2. Waldmann T. 1991. J. Biol. Chem. 266:2681.
Gene ID: 3559
UniProt: View information about CD25 on UniProt.org
Clone: BC96
Regulatory Status: RUO
Workshop: V T-072
Other Names: Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype: Mouse IgG1, κ