Biolegend, 303007, APC anti-human CD29 Antibody, 25tests

CD29 is a 130 kD single chain type I glycoprotein also known as integrin β 1 , VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen tissue sections3,5, and activation of integrin ß14,7,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.10
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208. Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP) Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ) Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) Murga M, et al. 2005. Blood 105:1992. (FC) PubMed Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ) Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ) Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity) Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)
Product Citations: He Y, et al. 2023. Protein Cell. 14:202. PubMed Yen FC, et al. 2023. Nat Commun. 14:2942. PubMed Benjamin DI, et al. 2022. Cell Metab. 34:902. PubMed Park S, et al. 2022. Biomolecules. 12: . PubMed McCurdy S, et al. 2022. FASEB J. 36:e22629. PubMed Gilazieva Z, et al. 2022. Curr Issues Mol Biol. 44:5363. PubMed Wei X, et al. 2022. Mol Cell. 82:1225. PubMed Mancuso RV, et al. 2020. British Journal of Pharmacology. 177(12):2696-2711.. PubMed Guney-Esken G, et al. 2021. Stem Cell Res Ther. 12:287. PubMed Wang T, et al. 2019. Mol Med Rep. 19:4719. PubMed Zhou X, et al. 2020. Cells. 9(4):. PubMed Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed Kang DH, et al. 2021. Nat Commun. 12:5059. PubMed Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed Sarkar TJ, et al. 2020. Nat Commun. 11:1545. PubMed Frazier T, et al. 2018. Adipocyte. 0.41875. PubMed Judson RN et al. 2018. Cell stem cell. 22(2):177-190 . PubMed Quarta M, et al. 2016. Nat Biotechnol. 10.1038/nbt.3576. PubMed Liu B, et al. 2017. Hum Cell. 30:51. PubMed Vishnoi M, et al. 2015. Sci Rep. 5: 17533. PubMed Velázquez–Avila M, et al. 2019. Leukemia. 33:1337. PubMed Yang C et al. 2019. Int J Mol Med. 43(3):1395-1405 . PubMed Murga M, et al. 2005. Blood. 105:1992. PubMed Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed Kitaeva KV, et al. 2022. Bioengineering (Basel). 9:. PubMed Guney-Esken G, et al. 2021. Methods Mol Biol. 2549:23. PubMed Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed Liao HQ, et al. 2021. Am J Reprod Immunol. 86:e13406. PubMed Huijskens M, et al. 2014. J Leukoc Biol. 96:1165. PubMed Ding S, et al. 2018. Sci Rep. 8:10808. PubMed Ruan Y, et al. 2022. Exp Ther Med. 23:47. PubMed Lu H, et al. 2020. Am J Reprod Immunol. 83:. PubMed Tabebordbar M, et al. 2021. Cell. 184:4919. PubMed Chen G, et al. 2015. J Endod. 41: 1091-1099. PubMed
RRID: AB_314323 (BioLegend Cat. No. 303007) AB_314324 (BioLegend Cat. No. 303008)
Structure: Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also associates with CD51 (αV), and α7- α9, 130 kD
Distribution: Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells
Function: Cell-cell and cell-matrix interactions
Ligand/Receptor: VCAM-1, MAdCAM-1, ECM
Cell Type: Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Hemler M. 1990. Annu. Rev. Immunol. 8:365. 2. Hynes R. 1992. Cell 69:11.
Gene ID: 3688
UniProt: View information about CD29 on UniProt.org
Clone: TS2/16
Regulatory Status: RUO
Workshop: V A-S202
Other Names: Integrin β1 chain, VLA-β chain, gpIIa, ITGB1
Isotype: Mouse IgG1, κ