Biolegend, 303407, APC anti-human CD33 Antibody, 25tests

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
25tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human myeloid leukaemia cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).
Application References(PubMed link indicates BioLegend citation): Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB) Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
Product Citations: Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed Harder L, et al. 2013. J Exp Med. 210:2289. PubMed Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed Frame JM, et al. 2020. Developmental Cell. 55(2):133-149.e6. PubMed Grenga I, et al. 2016. Clin Transl Immunology. 0.265972222. PubMed Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed Maat H, et al. 2021. iScience. 24(5):102435. PubMed Abd Hamid M, et al. 2020. Cancer Immunology Research. 8(2):203-216. PubMed Arvindam US, et al. 2021. Leukemia. 35:1586. PubMed Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed Burel JG, et al. 2019. Elife. 8. PubMed
RRID: AB_314351 (BioLegend Cat. No. 303407) AB_314352 (BioLegend Cat. No. 303408)
Structure: Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution: Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
Function: Adhesion and lectin activity
Ligand/Receptor: Sugar chains containing sialic acid
Cell Type: B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Siglec Molecules
Antigen References: 1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. 2. Freeman S, et al. 1995. Blood 85:2005.
Gene ID: 945
UniProt: View information about CD33 on UniProt.org
Clone: WM53
Regulatory Status: RUO
Workshop: IV M-505
Other Names: Siglec-3, gp67, p67
Isotype: Mouse IgG1, κ