Biolegend, 304824, PerCP/Cyanine5.5 anti-human CD62L Antibody, 100tests

CD62L is a 74-95 kD single chain type I glycoprotein referred to as L-selectin or LECAM-1. It is expressed on most peripheral blood B cells, subsets of T and NK cells, monocytes, granulocytes, and certain hematopoietic malignant cells. CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. CD62L is important for the homing of naïve lymphocytes to high endothelial venules in peripheral lymph nodes and Peyer's patches. It also plays a role in leukocyte rolling on activated endothelial cells.
100tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee, Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Concentrated supernatant from PMA-activated human peripheral blood leukocytes
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting2,3,9 and in vitro blocking of lymphocytes binding to high endothelial venules (HEV)2. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 304853-304858).
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. Kishimoto TK, et al. 1990. Proc. Natl. Acad. Sci. USA 87:2244. (WB, Block) Jutila M, et al. 2002. J. Immunol. 169:1768. (WB) Tamassia N, et al. 2008. J. Immunol. 181:6563. (FC) PubMed Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Koenig JM, et al. 1996. Pediatr. Res. 39:616. (WB) Shi C, et al. 2011. J. Immunol. 187:5293. (FC) PubMed Burges M, et al. 2013. Clin Cancer Res. 19:5675. PubMed Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Product Citations: Arasanz H, et al. 2022. Cancers (Basel). 14: . PubMed Cheng H, et al. 2023. Nat Metab. 5:314. PubMed Weulersse M, et al. 2020. Immunity. 53(4):824-839.e10. PubMed Chen GM, et al. 2021. Cancer Discov. 11:2186. PubMed Todd J, et al. 2016. PLoS Med. 13:e1002139. PubMed Ono-Moore K, et al. 2016. J Nutr. 146: 1411 - 1419. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Li H, et al. 2021. Nat Commun. 12:2773. PubMed Putz EJ, et al. 2019. J Dairy Sci. 102:9268. PubMed Putz EJ, et al. 2020. BMC Vet Res. 0.9. PubMed Zhu S, et al. 2022. J Oncol. 2022:8724933. PubMed Lee HJ, et al. 2017. Oncotarget. 8:113345. PubMed Bourges C, et al. 2020. EMBO Mol Med. 12:e12112. PubMed Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed Coughlan A, et al. 2012. J Immunol Methods. 385:96. PubMed Trinité B, et al. 2014. PLoS One. 9:110719. PubMed Li L, et al. 2022. JCI Insight. 7:. PubMed
RRID: AB_893396 (BioLegend Cat. No. 304823) AB_2239105 (BioLegend Cat. No. 304824)
Structure: Selectin, single chain glycoprotein, 74-95 kD
Distribution: Majority of B cells, naïve T cells, subset of memory T and NK cells, monocytes, granulocytes, thymocytes
Function: Leukocyte homing, leukocyte tethering, rolling
Ligand/Receptor: CD34, GlyCAM, MAdCAM-1
Cell Type: B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Thymocytes, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Kishimoto T, et al. 1990. P. Natl. Acad. Sci. USA 87:2244. Kishimoto T, et al. 1991. Blood 78:805.
Gene ID: 6402
UniProt: View information about CD62L on UniProt.org
Clone: DREG-56
Regulatory Status: RUO
Workshop: V S056
Other Names: L-selectin, LECAM-1, LAM-1, Leu-8, TQ-1
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.