Biolegend, 304909, APC anti-human CD62P (P-Selectin) Antibody, 25tests

CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.
25tests
Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4 and in vitro blocking of adhesion of platelets1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 & 304948).
Application References(PubMed link indicates BioLegend citation): Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block) Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Yen YT, et al. 2006. J. Virol. 80:2684. Sato Y, et al. 2005. Blood 106:428. (IHC)
Product Citations: Wu Y, et al. 2021. Immunity. 54:2595. PubMed Kemble S, et al. 2022. Blood Adv. 6:2932. PubMed Efendieva Z, et al. 2023. Sci Rep. 13:945. PubMed Johnson G, et al. 2013. Perfusion. 28:263. PubMed Malacrida B, et al. 2021. iScience. 24(6):102676. PubMed Ito Y, et al. 2018. Cell. 174:636. PubMed Eipper S, et al. 2016. PLoS One. 11: 0151509. PubMed Zheng TJ, et al. 2021. Am J Physiol Cell Physiol. 320:C902. PubMed Villarroel J, et al. 2013. J Surg Res. 184:422. PubMed Nam GS, et al. 2020. Int J Mol Med. 45:658. PubMed Kamat V, et al. 2015. Exp Hematol. . PubMed Inglis H, et al. 2015. J Vis Exp. 97: 52484. PubMed Li T, et al. 2022. J Clin Invest. 132:. PubMed Ashar H,et al. 2017. Am J Pathol.. 10.1016/j.ajpath.2017.09.014. PubMed
RRID: AB_314482 (BioLegend Cat. No. 304910)
Structure: Selectin, type I glycoprotein, 140 kD
Distribution: Activated platelets, megakaryocytes, endothelial cells
Function: Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
Ligand/Receptor: CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type: Endothelial cells, Megakaryocytes, Neutrophils, Platelets
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. McEver R, et al. 1995. J. Biol. Chem. 270:11025. 2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390.
Gene ID: 6403
UniProt: View information about CD62P on UniProt.org
Clone: AK4
Regulatory Status: RUO
Workshop: VI P-44
Other Names: GMP-140, PADGEM
Isotype: Mouse IgG1, κ