Biolegend, 305207, PE anti-human CD80 Antibody, 25tests

CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.
25tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC) Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB) Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations: Werz O, et al. 2018. Nat Commun. 9:59. PubMed Lah S, et al. 2023. J Immunother Cancer. 11:. PubMed Wang Y, et al. 2023. Hum Vaccin Immunother. 19:2202127. PubMed Nshimiyimana R, et al. 2023. Am J Hematol. 98:1000. PubMed Costa MHG, et al. 2021. Biotechnol J. 16:e2000389. PubMed Lertjuthaporn S, et al. 2022. Pathogens. 11: . PubMed Wu L, et al. 2023. Cell Rep Med. 4:100917. PubMed Lathrop S, et al. 2015. Infect Immun . 83: 2661 - 2671. PubMed Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed Rosskopf S, et al. 2016. Sci Rep. 6:31580. PubMed Hou ZH, et al. 2020. Am J Physiol Cell Physiol. 318:C649. PubMed Santiago K, et al. 2016. Pathog Dis. 74: ftw081. PubMed Du X, et al. 2018. Cell Res. 28:416. PubMed Herath S, et al. 2014. PLoS One. 9:88327. PubMed Wang D, et al. 2016. J Cell Sci. 129: 2261 - 2272. PubMed Guo Z, et al. 2022. NPJ Precis Oncol. 6:34. PubMed Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed Lewis SA, et al. 2021. Front Immunol. 12:724015. PubMed Balan I, et al. 2022. Front Immunol. 13:940095. PubMed Ganesan R, et al. 2020. FASEB J. 34:15888. PubMed Zhang L, et al. 2011. Blood. 117:6184. PubMed Blaszczak AM, et al. 2019. J Diabetes Res. 2019:8124563. PubMed Serhan J 2012. Blood. 120:e60. PubMed Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed Luque-Martin R, et al. 2021. J Immunol. 207:555. PubMed Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed Kubala MH, et al. 2018. Cell Rep. 25:2177. PubMed Gärtner K, et al. 2019. J Extracell Vesicles. 8:1573051. PubMed Longbrake EE, et al. 2018. Mult Scler. 24:728. PubMed Corgnac S, et al. 2022. J Immunother Cancer. 10:. PubMed Chen R, et al. 2021. Front Oncol. 11:743050. PubMed Morell M, et al. 2022. EBioMedicine. 76:103808. PubMed Trabanelli S, et al. 2014. J Immunol. 192:1231. PubMed
RRID: AB_314503 (BioLegend Cat. No. 305207) AB_314504 (BioLegend Cat. No. 305208)
Structure: Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution: Activated B cells and T cells, macrophages, and dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Freeman G, et al. 1991. J. Exp. Med. 174:625. 2. Linsley P, et al. 1996. Immunity 4:535. 3. Linsley P, et al. 1991. J. Exp. Med. 174:561.
Gene ID: 941
UniProt: View information about CD80 on UniProt.org
Clone: 2D10
Regulatory Status: RUO
Workshop: VI CD80.1
Other Names: B7-1, B7, BB1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.