Biolegend, 305217, PE/Cyanine7 anti-human CD80 Antibody, 25tests

CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.
25tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC) Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB) Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations: Avital G, et al. 2022. Cell Rep. 41:111477. PubMed Korniotis S, et al. 2022. J Cell Sci. 135:. PubMed García-García A, et al. 2023. Bioact Mater. 24:174. PubMed Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed Abe S, et al. 2020. JCI Insight. 5:00. PubMed Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed Kotsiou E, et al. 2016. Blood. 128: 72 - 81. PubMed Neff CP et al. 2018. EBioMedicine. 30:192-202 . PubMed Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed Sarkar MK, et al. 2018. Ann Rheum Dis. 77:1653. PubMed Boyd A, et al. 2013. Infect Immun . 81:1420. PubMed Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed Sabree SA, et al. 2021. J Immunother Cancer. 9: . PubMed Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed Cecotto L, et al. 2022. iScience. 25:105211. PubMed Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed Murphy DM, et al. 2022. J Clin Invest. Online ahead of print. PubMed
RRID: AB_1877254 (BioLegend Cat. No. 305217) AB_2076148 (BioLegend Cat. No. 305218)
Structure: Ig superfamily, type I transmembrane glycoprotein, 60 kD
Distribution: Activated B cells and T cells, macrophages, and dendritic cells
Function: T cell costimulation
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Freeman G, et al. 1991. J. Exp. Med. 174:625. 2. Linsley P, et al. 1996. Immunity 4:535. 3. Linsley P, et al. 1991. J. Exp. Med. 174:561.
Gene ID: 941
UniProt: View information about CD80 on UniProt.org
Clone: 2D10
Regulatory Status: RUO
Workshop: VI CD80.1
Other Names: B7-1, B7, BB1
Isotype: Mouse IgG1, κ