Biolegend, 305534, StarBright UltraViolet 795 anti-human CD94 Antibody, 100tests

This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For ordering assistance or to request a sample, contact your local field representative . This product is also available for direct purchase within a StarBright Basic Panel , which combines a minimal set of pre-selected compatible reagents. CD94 is a 43 kD type II transmembrane glycoprotein also known as KP43. CD94 belongs to the C-type lectin superfamily and is present as a covalently linked heterodimer with NKG2 on the cell surface. CD94 is expressed by NK cells, a subset of γδ T cells, and NKT cells. The CD94/NKG2A complex serves as an inhibitory receptor specific for HLA-class I molecules.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NK cell line
Formulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 795 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) The concentration relates to the dye component of the conjugate.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * StarBright UltraViolet 795 has a maximum excitation of 340 nm and a maximum emission of 792 nm. StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser: Ultraviolet Laser (355 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation4, inhibition of NK cell-mediated lysis5, immunohistochemical staining of acetone-fixed frozen tissue sections, and spatial biology (IBEX)6,7.
Application References(PubMed link indicates BioLegend citation): Mizuki M, et al. 2000. Sarcoidosis Vasc. Diffuse Lung Dis. 17:54. Phillip J, et al. 1996. Immunity 5:163. Lazetic S, et al. 1996. J. Immunol. 157:4741. Lanier LL, et al. 1998. Immunity 8:693. Wooden SL, et al. 2005. J. Immunol. 175:1383. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Structure: C-type lectin, type II transmembrane glycoprotein, covalently associates with NKG2, 43 kD
Distribution: NK cells , T subset
Function: Inhibits NK function
Cell Type: NK cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Lopez-Botet M, et al. 1997. Immunol. Rev. 155:165. 2. Moretta A, et al. 1997. Immunol. Rev. 155:105.
Gene ID: 3824
UniProt: View information about CD94 on UniProt.org
Clone: DX22
Regulatory Status: RUO
Other Names: KP43
Isotype: Mouse IgG1, κ
Q: What is a “StarBright panel”?
A: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.
Q: Will my StarBright panel be provided as a cocktail?
A: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.
Q: Are StarBright GMP/ASR products available?
A: StarBright ASR and GMP products are coming soon.
Q: Are StarBright custom products available?
A: Yes, please contact our Custom Solutions Team.
Q: Will StarBright isotype controls be offered?
A: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.
Q: What amount of a StarBright conjugate should I use?
A: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.
Q: Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?
A: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.