Biolegend, 306510, APC anti-human CD184 (CXCR4) Antibody, 100tests

CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2.
100tests
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon, Chimpanzee, Sooty Mangabey
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CP-MAC-infected Sup-T1 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10 The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).
Application References(PubMed link indicates BioLegend citation): McKnight A, et al. 1997. J. Virol. 71:1692. Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF) Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC) Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block) Ullrich CK, et al. 2000. Blood 96:1438. (Block) Murga M, et al. 2005. Blood 105:1992. (IF) Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed Isnardi I, et al. 2010. Blood 115:5026. PubMed Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Fischer T, et al. 2008. PLoS One 3:e4069. Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)
Product Citations: Gao Y, et al. 2016. Nat Methods. 10.1038/nmeth.4042. PubMed Matkovic Leko I, et al. 2023. Nat Protoc. . PubMed Ellis GI, et al. 2022. Cell Rep Med. 3:100614. PubMed Lu D, et al. 2022. ACS Synth Biol. 11:1397. PubMed Biasci D, et al. 2020. Proc Natl Acad Sci U S A. 117:28960. PubMed Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed Xu X, et al. 2021. Molecular Cell. :. PubMed Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed Isnardi I, et al. 2010. Blood. 115:5026. PubMed Gludish DW, et al. 2020. Sci Rep. 10:19900. PubMed Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed McQuade A, et al. 2020. Nat Commun. 4.1875. PubMed Agha O, et al. 2020. JOR Spine. 3:e1091. PubMed Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed Roukens AHE, et al. 2022. Nat Immunol. 23:23. PubMed Hyun Hee Lee, Vanessa Bellat, Benedict Law 2017. PLoS One. 12(2):e0171044. PubMed Huijbregts R, et al. 2013. Endocrinology. 154:1282. PubMed Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed Lu P, et al. 2021. Blood Cancer J. 11:39. PubMed Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed Cai J, et al. 2021. eLife. 10:00. PubMed Rai MA, et al. 2020. Retrovirology. 17:35. PubMed Kempton HR, et al. 2020. Mol Cell. 184:78. PubMed Fiedorowicz M, et al. 2020. Sci Rep. 10:5379. PubMed Foight GW, et al. 2019. Nat Biotechnol. 37:1209. PubMed Bourges C, et al. 2020. EMBO Mol Med. 12:e12112. PubMed Suzuki S, et al. 2021. STAR Protocols. 2:100683. PubMed Toyoda M, et al. 2015. J Virol. 89: 9639-9652. PubMed Shi J, et al. 2012. Blood. 120:1624. PubMed Meng YM, et al. 2018. Oncoimmunology. 7:e1408745. PubMed Walker KL, et al. 2021. Leuk Lymphoma. 62:1167. PubMed Grosche L, et al. 2020. Viruses. 12:. PubMed Blanco-Camarillo C, et al. 2021. Front Immunol. 12:672520. PubMed Ogaki S, et al. 2015. Sci Rep. 5:17297. PubMed Nakamura M, et al. 2019. Nat Commun. 10:194. PubMed Corgnac S, et al. 2022. J Immunother Cancer. 10:. PubMed Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed Samuel RM, et al. 2020. Cell Stem Cell. 27:876. PubMed Shan L, et al. 2017. Immunity. 47:766. PubMed Rodrigues Toste de Carvalho AL, et al. 2021. Nat Protoc. 16:1802. PubMed Olety B, et al. 2021. Sci Adv. 7:eabj7398. PubMed
RRID: AB_314615 (BioLegend Cat. No. 306509) AB_314616 (BioLegend Cat. No. 306510)
Structure: Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD
Distribution: T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells
Function: B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development
Ligand/Receptor: SDF-1 receptor, coreceptor for X4 HIV-1
Cell Type: B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657. 2. Loetscher P, et al. 2000. Adv. Immunol. 74:127. 3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.
Gene ID: 7852
UniProt: View information about CD184 on UniProt.org
Clone: 12G5
Regulatory Status: RUO
Workshop: VII 70204
Other Names: CXCR4, Fusin
Isotype: Mouse IgG2a, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.