Biolegend, 307006, PE anti-human DcR1 (TRAIL-R3, CD263) Antibody, 100tests

DcR1 is a 65 kD GPI-anchored member of the TNFR superfamily (TNFRSF10C) also known as TRAIL-R3, TRID, CD263, and LIT. Although not expressed by most tumors, DcR1 is expressed at low levels on normal tissues, acting as a TRAIL decoy receptor, thus inhibiting apoptosis. DcR1 is termed a decoy receptor, as it does not have a functional death domain to induce apoptosis following TRAIL ligation.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Extracellular domain of DcR1-human IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: The DJR3 antibody is useful for immunofluorescent staining and flow cytometric analysis of DcR1/TRAIL-R3 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307004) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).
Application References(PubMed link indicates BioLegend citation): Uno K, et al. 2003. Blood 101:3658. Sato K, et al. 2005. J. Immunol. 174:4025.
Product Citations: Greenlee JD, et al. 2021. eLife. 0.416666666666667. PubMed Chen QZ, et al. 2020. Front Immunol. 1.202777778. PubMed Staniek J, et al. 2019. Front Immunol. 10:951. PubMed
RRID: AB_2287506 (BioLegend Cat. No. 307005) AB_2205089 (BioLegend Cat. No. 307006)
Structure: TNFR superfamily, GPI-anchored, truncated death domain, 65 kD
Distribution: Most normal tissues, not tumors
Function: TRAIL decoy, inhibits apoptosis
Ligand/Receptor: TRAIL
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Schneider P, et al. 1997. J. Biol. Chem. 272:25417. 2. Pan G, et al. 1997. Science 277:815.
Gene ID: 8794
UniProt: View information about CD263 on UniProt.org
Clone: DJR3
Regulatory Status: RUO
Other Names: TRAIL-R3, TRID, LIT, CD263, TNFRSF10C
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.