Biolegend, 308106, PE anti-human Perforin Antibody, 100tests

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
100tests
Verified Reactivity: Human
Reported Reactivity: Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified granules from the human lymphoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone dG9 primarily recognizes perforin associated with cytotoxic granules9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.Does not cross-react with mouse1.
Application References(PubMed link indicates BioLegend citation): Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC) Schaerli P, et al. 2004. J. Exp. Med. 199:1265. Watanabe N, et al. 1997. Blood 90:3662. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC) Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF) Chen H, et al. 2005. J. Immunol. 175:591. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF) Wood SM, et al. 2009. Blood 114:4117. PubMed Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.
Product Citations: Woan KV, et al. 2021. Cell Stem Cell. 28:2062. PubMed Tang-Huau TL, et al. 2018. Nat Commun. 9:2570. PubMed Seo IH, et al. 2021. Immune Netw. 21:e17. PubMed Hejazi M, et al. 2022. Front Immunol. 12:798087. PubMed Su S et al. 2018. Cell. 175(2):442-457 . PubMed Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed Lettau M, et al. 2018. Int Immunol. 30:215. PubMed Georg P, et al. 2022. Cell. 185:493. PubMed Kuttruff S, et al. 2009. Blood. 113:358. PubMed Van der Meer JM, et al. 2020. Cancer Immunol Immunother. . PubMed Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed Bertino P, et al. 2014. Vaccine. 32:1670. PubMed
RRID: AB_314703 (BioLegend Cat. No. 308105) AB_314704 (BioLegend Cat. No. 308106)
Structure: 70 kD
Distribution: CTL, NK (cytoplasmic granules)
Function: Mediates targeted cell lysis
Cell Type: NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361. 2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
Gene ID: 5551
UniProt: View information about Perforin on UniProt.org
Clone: dG9
Regulatory Status: RUO
Other Names: PRF1, P1, PFP, HPLH2
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.