Biolegend, 308112, APC anti-human Perforin Antibody, 100tests

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
100tests
Verified Reactivity: Human
Reported Reactivity: Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified granules from the human lymphoma cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone dG9 primarily recognizes perforin associated with cytotoxic granules9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.Does not cross-react with mouse1.
Application References(PubMed link indicates BioLegend citation): Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC) Schaerli P, et al. 2004. J. Exp. Med. 199:1265. Watanabe N, et al. 1997. Blood 90:3662. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC) Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF) Chen H, et al. 2005. J. Immunol. 175:591. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF) Wood SM, et al. 2009. Blood 114:4117. PubMed Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.
Product Citations: Balta E, et al. 2023. Front Immunol. 13:1063313. PubMed Jung IY, et al. 2022. Sci Transl Med. 14:eabn7336. PubMed Wang F, et al. 2018. Oncogenesis. 7:41. PubMed Myers JA, et al. 2022. JCI Insight. :. PubMed Jilek S, et al. 2012. J Immunol. 188:4671. PubMed Jie HB, et al. 2017. Cancer Immunol Res. 0.491666667. PubMed Grogan B, et al. 2011. Biol Blood Marrow Transplant. 17:1121. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Hirschberger S, et al. 2022. Front Med (Lausanne). 9:923502. PubMed Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed Shen C, et al. 2021. BMC Med. 19:283. PubMed Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
RRID: AB_830872 (BioLegend Cat. No. 308111) AB_2252843 (BioLegend Cat. No. 308112)
Structure: 70 kD
Distribution: CTL, NK (cytoplasmic granules)
Function: Mediates targeted cell lysis
Cell Type: NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361. 2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
Gene ID: 5551
UniProt: View information about Perforin on UniProt.org
Clone: dG9
Regulatory Status: RUO
Other Names: PRF1, P1, PFP, HPLH2
Isotype: Mouse IgG2b, κ