Biolegend, 308306, PE anti-human CD255 (TWEAK) Antibody, 100tests

TNF-like weak inducer of apoptosis (TWEAK) is a type II transmembrane TNF superfamily member with strong homology to TNF in its extracellular portion, though less potent in inducing apoptosis. It has been clustered as CD255. TWEAK transcript is expressed broadly in many adult and fetal tissues; however, the staining of human peripheral blood mononuclear cells with TWEAK-specific antibodies shows a more restricted pattern. TWEAK is expressed as membrane-bound and secreted forms. Interaction of TWEAK with its receptors (DR3, Fn14) promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, and apoptosis in a number of human cell lines.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: hTWEAK/2PK-3 transfectant cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Flow Cytometry: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 308304) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)). ELISA Detection: The biotinylated CARL-1 antibody is useful as the detection antibody in sandwich ELISA for measuring human TWEAK protein, in combination with CARL-2 antibody (Cat. No. 308402) used as ELISA capture. Neutralization1,2: In a dose-dependent manner, CARL-1 is more effective than CARL-21. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 308307).
Application References(PubMed link indicates BioLegend citation): Nakayama, M., et al. 2000. J. Exp. Med. 192:1373. (Neut) Nakayama, M., et al. 2003. J. Immunol. 170:341. (Neut) Zou H, et al. 2011. Gynecol Oncol.123:123. PubMed.
Product Citations: Alvarez RA, et al. 2014. J Virol. 6031:88. PubMed Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed
RRID: AB_314709 (BioLegend Cat. No. 308305)
Structure: TNF superfamily; 17 kD (Mammalian)
Distribution: Normal tissue and tumor cells at low levels
Function: Promotes secretion of IL-8, activation of NF-κB, proliferation of endothelial cells, apoptosis
Bioactivity: Weak apoptotic induction; mediate CD4+ T-cell killing of macrophages; induces IL-6, IL-8, ICAM-1 in astrocytes; HUVEC proliferation
Cell Sources: Transcripts widely expressed and abundant in most tissues; protein efficiently secreted
Cell Targets: Macrophages, astrocytes, endothelial cells
Receptors: Apo-3 (DR3, WSL-1, TRAMP, LARD); TWEAKR
Biology Area: Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: CD Molecules, Cytokines/Chemokines
Antigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Chicheportiche, Y., et al. 1997. J. Biol. Chem. 272:32401. 3. Kaplan, M., et al. 2000. J. Immunol. 164:2897. 4. Saas, P., et al. 2000. Glia 32:102. 5. Wiley, S., et al. 2001. Immunity 15:837.
Regulation: Type II integral membrane protein readily cleaved from cell surface
Gene ID: 8742
UniProt: View information about CD255 on UniProt.org
Clone: CARL-1
Regulatory Status: RUO
Other Names: TNF-like weak inducer of apoptosis, Apo-3 ligand (Apo3L), Death receptor 3-L (DR3-L), TNFSF 12, CD255
Isotype: Mouse IgG3, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.